Do you use antibody fragments? Check out Andrew Kinman's systematic, thoughtful, and very useful protocol for generating fragments at tiny (microgram) scales by pepsin cleavage, now published in Bioconjugate Chemistry. This work should save a lot of time for researchers who fragment antibodies for any purpose.
Email the group:
pompanogroup@virginia.edu
Lab address for postal mail:
Department of Chemistry
University of Virginia
PO BOX 400319
Charlottesville, VA22904
Address for shipments:
409 McCormick Road
University of Virginia
Charlottesville, VA22904
Lab phone number:
(434) 924-7723
Lab physical location:
Chemistry 107
See Getting Here for a map and directions to the Chemistry building.
Lab summit, Feb 2020
Group Picture, 2016