All Publications, sorted by date
Click the links above to view papers in specific categories.
For reprints of any of these publications, please email Dr. Pompano: rpompano@virginia.edu. Any PDFs are provided solely for personal or educational use. We are fortunate to have several stellar undergraduate researchers in the lab. (#) Indicates undergraduate co-author.
A list of all peer reviewed publications can be found at Prof. Pompano’s Google Scholar page.
We now have a free, public UVA DataVerse archive, including representative datasets and microdevice design files, since 2023.
For articles affiliated with American Chemical Society (ACS), a link to view the article without a paywall is provided. Use of the link requires a free account with ACS in order to view the full article. Tip: ACS account passwords must include all three of the following symbols: “_” “.” “@”
Rebecca R. Pompano. "Boosting B cells in blood-derived organoids." Nature Materials (2025). https://doi.org/10.1038/s41563-024-02113-6
Katerina Morgaenko, Abhinav Arneja, Alexander G. Ball, Audrey M. Putelo, Jennifer M. Munson, Melanie R. Rutkowski, Rebecca R. Pompano, “Ex vivo model of breast cancer cell invasion in live lymph node tissue.” ACS Pharmacology & Translational Science, 2025, ASAP article. [Open access at publisher]
Jonathan M Zatorski*, Djuro Raskovic*, Abhinav Arneja, Saweetha Kiridena, Tochukwu Ozulumba, Jennifer H Hammel, Parastoo Anbaei, Jennifer E Ortiz-Cárdenas, Thomas J Braciale, Jennifer Munson, Chance John Luckey, Rebecca R Pompano. “Initiation of primary T cell—B cell interactions and extrafollicular antibody responses in an organized microphysiological model of the human lymph node.” bioRxiv 2025.01.12.632545 [Preprint available, prior to peer review]
*Equal contributions
Jennifer H Hammel, Abhinav Arneja, Jessica Cunningham, Maosen Wang, Sophia Schumaecker, Yamilet Macias Orihuela, Tochukwu Ozulumba, Jonathan Zatorski, Thomas J Braciale, Chance John Luckey, Rebecca R Pompano, Jennifer M Munson, “Engineered human lymph node stroma model for examining interstitial fluid flow and T cell egress.” bioRxiv 2024.12.03.622729. [Preprint available, prior to peer review]
Jonathan M. Zatorski*, (#)Isabella L. Lee*, Jennifer E. Ortiz-Cárdenas, Jeffrey F. Ellena, Rebecca R. Pompano. “Measuring covalent bond formation in light-curing hydrogels predicts stability under fluid flow,” Analytical Chemistry, in press. [Open access at publisher] *Equal contributions.
Sophie R. Cook, Alexander G. Ball, Anwaruddin Mohammad, Rebecca R. Pompano. “A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node.” Lab on a Chip, 2025, in press. DOI: 10.1039/D4LC00489B [Open access at publisher]
Alexander G. Ball, Katerina Morgaenko, Parastoo Anbaei, Sarah E. Ewald, Rebecca R. Pompano. “Poly I:C vaccination drives transient CXCL9 expression near B cell follicles in the lymph node through type-I and type-II interferon signaling.” Cytokine, 2024, 183, 156731. [Publisher link]
Tochukwu Ozulumba, Jonathan M. Zatorski, Abhinav Arneja, Jennifer H. Hammel, Thomas J. Braciale, Chance John Luckey, Jennifer M. Munson, Rebecca R. Pompano. “Mitigating reactive oxygen species production and increasing gel porosity improves lymphocyte viability and fibroblast spreading in photocrosslinked gelatin-thiol hydrogels.” In revision. [BioRxiv preprint available, prior to peer review]
Parastoo Anbaei, Marissa G. Stevens, Alexander G. Ball, Timothy N.J. Bullock, Rebecca R. Pompano, “Spatially resolved quantification of oxygen consumption rate in ex vivo lymph node slices” Analyst, 2024, 149, 2609-2620. [Open access at publisher]
Selected as an Analyst HOT Article of 2024
Hannah B. Musgrove, Sophie R. Cook, and Rebecca R. Pompano. “Parylene-C coating protects resin 3D printed devices from materials erosion and cytotoxicity.” ACS Applied Bio Materials, 2023, 6, 8, 3079–3083 [Publisher link] [ChemRxiv][Design Files]
Ozulumba T, Montalbine AN (#), Ortiz-Cárdenas JE, Pompano RR. “New tools for immunologists: Experimental and computational models of lymph node function from cell to tissue levels”. Frontiers in Immunology, 2023, 14, doi: 10.3389/fimmu.2023.1183286. Review article, open access.
Musgrove HB, Saleheen A, Zatorski JM, Arneja A, Luckey CJ, and Pompano RR. “A scalable, modular degasser for passive in-line removal of bubbles from biomicrofluidic devices.” Micromachines, 2023, 14(2), 435. [Open access at publisher][design files]
Featured in the Women’s Special Issue Series: Micromachines.
Musgrove HB and Pompano RR. “Threadless chip-to-world connections on resin 3D printed microscale devices.” In Chips & Tips (2022), a curated open access blog associated with Lab on a Chip. [design files]
Hannah B. Musgrove, Megan A. Catterton, Rebecca R. Pompano, “Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing.” Analytica Chimica Acta, 2022, 339842. [publisher link]. [Download: ZIP file of CAD drawings]
Featured in special issue: 3D Printing for Analytical Chemistry
View similar version at BioRxiv.
Harris AR, Esparza S, Azimi MS, Cornelison R, Azar FN, Llaneza DC, Belanger M, Mathew A, Tkachenko S, Perez MJ, Rosean CB, Bostic RR, Cornelison RC, Tate KM, Peirce-Cottler SM, Paquette C, Mills A, Landen CN, Saucerman J, Dillon PM, Pompano RR, Rutkowski MA and Munson JM. “Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented with Adjuvant Anti-VEGFR3 Therapy.” Frontiers in Oncology, 2022, 12. [Publisher link]
Ortiz-Cárdenas JE, Zatorski JM, Arneja A, Montalbine AN (#), Munson JM, Luckey CJ, & Pompano RR. “Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device.” Organs-on-a-Chip, 2022, 4, 100018 [Publisher link]
Featured in Emerging Investigators in Organs-on-a-Chip special issue.
Sophie R. Cook, Hannah B. Musgrove, Amy L. Throckmorton, & Rebecca R Pompano. “Microscale impeller pump for recirculating flow in organs-on-chip and microreactors.” Lab on a Chip, 2022. 2022, 22, 605 - 620. {Publisher link]
Jennifer H. Hammel, Jonathan M. Zatorski, Sophie R. Cook, Rebecca R. Pompano, Jennifer M. Munson. “Engineering in vitro immune-competent tissue models for testing and evaluation of therapeutics,” Advanced Drug Delivery Reviews (2022). 182, 114111. [Publisher link]
Hunter RA, Pompano RR, & Tuchler MF. “Alternative Assessment of Active Learning,” Chapter 15 in ACS Symposium Series: Active Learning in the Analytical Chemistry Curriculum. 2022. Editors: Wenzel T, Kovarik M, Robinson J. pp. 269-295. DOI: 10.1021/bk-2022-1409.ch015. Online before print.
Invited contribution.
Megan A. Catterton, Alexander G. Ball, and Rebecca R. Pompano. "Rapid Fabrication by Digital Light Processing 3D Printing of a SlipChip with Movable Ports For Local Delivery to Ex Vivo Organ Cultures" Micromachines (2021) 12, no. 8: 993. https://doi.org/10.3390/mi12080993 [Link, open access]
Catterton M, Montalbine AN (#), & Pompano RR. “Selective Fluorination of the Surface of Polymeric Materials After Stereolithography 3D Printing.” Langmuir (2021) 37, 24, 7341–7348. [Publisher link]
Affiliated with ACS.
JH Hammel’, SR Cook’, MC Belanger’, JM Munson, and RR Pompano. “Modeling Immunity In Vitro: Slices, Chips, and Engineered Tissues.” Annual Review of Biomedical Eng, 2021, online ahead of print. [pubmed] ‘Equal contributions.
Venton BJ and Pompano RR. “Strategies for enhancing remote student engagement through active learning.” Analytical and Bioanalytical Chemistry (2021), online before print. [Link]
Invited contribution.
View the entire series featuring teaching analytical science during the pandemic.
Maura C. Belanger, Alexander G. Ball*, Megan A. Catterton*, Andrew W.L. Kinman*, Parastoo Anbaei, Benjamin D. Groff (#), Stephanie J. Melchor, John R. Lukens, Ashley E. Ross, & Rebecca R. Pompano. “Acute lymph node slices are a functional model system to study immunity ex vivo.”ACS Pharmacology & Translational Science (2021), 4, 1, 128–142. *Equal contributions. [Publisher link] [Pubmed Central]
Ball AG, Belanger MC, & Pompano RR. “Detergent wash improves vaccinated lymph node handling ex vivo” J Immunological Methods, 2021, 112943. [Publisher link] [final version on BioRxiv]
Austin F. Dunn, Megan A. Catterton, Drake D. Dixon(#), and Rebecca R. Pompano. “Spatially resolved measurement of dynamic glucose uptake in live ex vivo tissues.” Analytica Chimica Acta (2021), 1141, 47-56. [Journal Link]
Prof. Pompano has shared a number of activities that she created for her analytical chemistry classroom in the ASDL Shorter Activities collection. Topics include:
Intro to Analytical Chem (adopted from Prof. Jill Venton)
Potentiometry
Fick’s laws of diffusion
Literature Article Analysis: Microfluidic Field Assays
Absorbance and fluorescence analysis: NAD(H) case study
Immunoassays, antibody immobilization
Dozens of other activities are also available on this site! An excellent resource for educators.
A small silver lining of the remote learning period during the COVID-19 pandemic is that Prof. Pompano recorded a number of lessons on analytical chemistry and bioanalytical chemistry and shared them publicly. New videos continue to be posted occasionally as needed for updated courses.
Bioanalytical Methods: Topics include antibodies as reagents, antibody binding, homogeneous and competitive immunoassays, and readouts such as flourescence and flow cytometry.
Introductory Analytical Chemistry: Topics include quanititative figures of merit, and intro to instrumentation for atomic spectroscopy, UV-Vis, and mass analyzers for mass spectrometry.
Belanger MC, Anbaei P, Dunn AF, Kinman AWL, and Pompano RR. “Spatially resolved analytical chemistry in intact, living tissues.” Analytical Chemistry, (2020) 92, 23, 15255–15262. [link]
Affiliated with ACS.
Zatorski JM, Montalbine AN (#), Ortiz-Cárdenas JE, & Pompano RR. “Quantification of fractional and absolute functionalization of gelatin hydrogels by optimized ninhydrin assay and 1H NMR.” Analytical and Bioanalytical Chemistry (2020), 412, 6211–6220. [Link]
Selected by the Editor as a “Paper in Forefront.”
Featured in the ABC Female Role Models in Analytical Chemistry special issue.
Work performed performed prior to UVA
Development of chemically-defined, peptide-based nanoparticles as non-inflammatory vaccines
Si Y, Wen Y, Chen J, Pompano RR, Han H, Collier JH, & Chong AS. “MyD88 in antigen-presenting cells is not required for CD4+ T-cell responses during peptide nanofiber vaccination,” MedChemComm (2018), 9, 138-148. DOI: 10.1039/C7MD00367F
Mora-Solano C, Wen Y, Han H, Chen J, Chong AS, Miller ML, Pompano RR, & Collier JH. "Active immunotherapy for TNF-mediated inflammation using self-assembled peptide nanofibers," Biomaterials (2017), 149, 1–11. [Pubmed]
Pompano RR*, Chen J*, Verbus EA (#), Han H, Fridman A, McNeely T, Collier JH, & Chong AS. “Titrating T-Cell Epitopes within Self-Assembled Vaccines Optimizes CD4+ Helper T Cell and Antibody Outputs,” Advanced Healthcare Materials (2014), 3(11): 1898-908. *Equal contributions. [PubMed] [PDF]
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Chen J*, Pompano RR*, Santiago FW, Maillat L, Sciammas R, Sun T, Han J, Topham DJ , Chong AS, & Collier JH “The use of self-adjuvanting nanofiber vaccines to elicit high-affinity B cell responses to peptide antigens without inflammation,” Biomaterials (2013) 34(34): 8776–8785. *Equal contributions. [PubMed]
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Purpose-driven microfluidic device design
Pompano RR, Platt CE (#), Karymov MA, & Ismagilov RF “Control of initiation, rate, and routing of capillary-driven flow of liquid droplets through microfluidic channels on SlipChip,” Langmuir (2012) 28(3): 1931-1941 [PubMed]
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Nichols KP, Pompano RR, Li L, Gelis AV, & Ismagilov RF "Toward Mechanistic Understanding of Nuclear Reprocessing Chemistries by Quantifying Lanthanide Solvent Extraction Kinetics via Microfluidics with Constant Interfacial Area and Rapid Mixing," J American Chemical Society (2011) 133(39):15721-15729. [Full Text]
Pompano RR, Liu W, Du W, & Ismagilov RF “Microfluidics Based On Spatially-Defined Arrays of Droplets in One, Two, and Three Dimensions,” Annual Review of Analytical Chemistry (2011) 4:59-81. (Review article) [Full Text]
Analysis of complex biochemical networks (blood clotting) using microfluidics and numerical simulations
Shen F, Pompano RR, Kastrup CJ & Ismagilov RF "Confinement Regulates Complex Biochemical Networks: Initiation of Blood Clotting by "Diffusion Acting"," Biophysical Journal (2009) 97, 2137-2145. [PubMed]
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Kastrup CJ, Boedicker JQ, Pomerantsev AP, Moayeri M, Bian Y, Pompano RR, Kline TR, Sylvestre P, Shen F, Leppla SH, Tang WJ & Ismagilov RF "Spatial localization of bacteria controls coagulation of human blood by 'quorum acting'," Nature Chemical Biology (2008) 4, 742-750. [PubMed]
Prior research interests
Russell LE, Pompano RR, Kittredge KW & Leopold MC "Assembled nanoparticle films with crown ether-metal ion "sandwiches" as sensing mechanisms for metal ions," J of Materials Science (2007) 42, 7100-7108. [Full Text]
Pompano RR, Wortley PG, Moatz LM, Tognarelli DJ, Kittredge KW & Leopold MC "Crown ether-metal "sandwiches'" as linking mechanisms in assembled nanoparticle films," Thin Solid Films (2006) 510, 311-319. [Full Text]
Tognarelli DJ, Miller RB, Pompano RR, Loftus AF, Sheibley DJ & Leopold MC "Covalently networked monolayer-protected nanoparticle films," Langmuir (2005) 21, 11119-11127. [Full Text]
Liu J, Shang XM, Pompano R & Eisenthal KB "Antibiotic assisted molecular ion transport across a membrane in real time," Faraday Discussions (2005) 129, 291-299.