Paper: Blue particles to label cells

Congratulations to Maura Belanger and Meng Zhuang from the Fraser lab for the publication of their work in Biomaterials Science. This project was initiated by talented undergraduate Kristen Richey several years ago.

We show that boron based, blue-fluorescent nanoparticles make good labels for endocytic immune cells in fluorescence imaging or flow cytometry. 

Take advantage of that underutilized blue channel on your microscope.

Advance article accessible here: https://pubs.rsc.org/en/content/articlepdf/2020/BM/C9BM01572H

Two-tissue microfluidic chip published

Congratulations to Dr. Sangjo Shim for his paper published his paper in Lab on a Chip, and featured in the Organ-, body-, and disease-on-a-chip thematic collection. This is the first microfluidic chip to connect two living tissues in a recirculating loop of media. We hope that this technology will enable many exciting studies of multi-tissue interactions. As a start, in collaboration with the Munson lab at Virginia Tech, he showed that it models one key feature of tumor immunity -- T cell immunosuppression.

See our Publications page for more.

Live immunostaining paper published

We are very proud of Andrew, Ben, and Jacob for publishing their method for fluorescent immunostaining of living lymph node tissue. Most available methods to label structures in tissue are intended for samples that have been fixed (i.e. killed). We showed that it is possible to get beautiful and informative images of tissue structure while the tissue is still alive, without perturbing key functions such as T cell activation. Check out their work in the Journal of Immunological Methods or on our Publications page.

Lab on a Chip paper published

Congratulations to Meg Catterton for putting out a great paper. You can read about her cool SlipChip for movable stimulation of lymph nodes and other tissue slices online today in Lab on a Chip.  This technology enables the user to move a microfluidic port to a desired location under a slice of tissue or 3D culture of cells, and deliver solution just to that spot.  We hope it will be useful for many other researchers who are interested in local events in live tissue.  Contact us if you are interested in testing it out!

User-defined local stimulation of live tissue through a movable microfluidic port

Analyst Cover

Our article entitled "Spatially resolved microfluidic stimulation of lymphoid tissue ex vivo" has been printed in The Analyst and has been featured on the cover! 

A PDF version of the full text of the publication can be accessed here.

Paper Publication!

Congrats to Dr. Ashley Ross, Maura Belanger, and Jacob Woodroof for publishing the first paper of the Pompano Lab.  The paper is entitled "Spatially resolved microfluidic stimulation of lymphoid tissue ex vivo" and can be found by clicking the link.  This paper is published in The Analyst.