Two papers were published from our lab in late 2020 and early 2021, establishing the methods and possibilities of using murine lymph node slices to study short-term adaptive immune responses.
Congratulations to Maura Belanger et al, for completing and publishing many years of work in ACS Pharmacology & Translational Science, 2021.
This work establishes our best practices for collection of murine lymph node slices, demonstrates a lack of overt inflammation from the process of slicing, and characterizes their ability to respond to direct and indirect T cell stimulation. Finally, it provides some of the first evidence that lymph node slices from vaccinated animals can response to antigen ex vivo. We look forward to continuing to share this platform with other researchers.
Congratulations to Alex Ball et al, who recently solved a longstanding bottleneck in our lab. We found that lymph nodes from vaccinated animals were the most difficult to slice, because did often failed to adhere to the supporting agarose hydrogel. Alex worked out a simple method to solve this problem, via a quick dip in a gentle detergent solution. We share this technical advance in the Journal of Immunological Methods, 2021.
Congratulations to Maura, Alex, and the entire team!
